RESEARCHER - M/F

About the company

Description THE CANDIDATE WILL JOIN MS. LAFFARGUE'S TEAM AT THE INSTITUTE OF METABOLIC AND CARDIOVASCULAR DISEASES (I2MC, UMR1297; HTTPS://WWW.I2MC.INSERM.FR (https://www.i2mc.inserm.fr)), LOCATED IN TOULOUSE. MS. LAFFARGUE'S TEAM IS INTERESTED IN THE RESPONSES OF VASCULAR CELLS INVOLVED IN ARTERIAL REMODELING LEADING TO THE DEVELOPMENT OF ATHEROSCLEROSIS AND TRANSPLANT VASCULOPATHY.

THE SUCCESSFUL CANDIDATE WILL JOIN THE TEAM LED BY DR. LAFFARGUE AT THE INSTITUTE OF METABOLIC AND CARDIOVASCULAR DISEASES (I2MC, UMR1297; HTTPS://WWW.I2MC.INSERM.FR (https://www.i2mc.inserm.fr/)), LOCATED IN TOULOUSE. DR. LAFFARGUE'S TEAM INVESTIGATES THE RESPONSES OF VASCULAR CELLS INVOLVED IN ARTERIAL REMODELING, WHICH CONTRIBUTES TO THE DEVELOPMENT OF ATHEROSCLEROSIS AND TRANSPLANT VASCULOPATHY.

Your tasks

Our research focuses on 1.the impact of mechanical stress on endothelial cell homeostasis,

2.new determinants of smooth muscle cell dysfunction, with a particular interest in the PI3K signaling pathway,

http://3.the preclinical and clinical validation of our studies. These studies aim to identify new cellular targets in order to propose new concepts and new therapeutic strategies to prevent arterial lesions.

THE CANDIDATE WILL BE RESPONSIBLE FOR THE MANAGEMENT AND EXECUTION OF PROJECTS AIMED AT CHARACTERIZING THE RESPONSE OF ENDOTHELIAL CELLS TO MECHANICAL STRESS IN A DISRUPTED METABOLIC CONTEXT. THEY WILL BE IN CHARGE OF ENSURING A COLLABORATIVE PROJECT AIMED AT ALSO UNDERSTANDING THIS RESPONSE IN A HYPOXIC CONTEXT. FINALLY, THE RECRUITED PERSON WILL CONTRIBUTE TO THE SCIENTIFIC VALORIZATION OF THE RESULTS OBTAINED AND WILL PARTICIPATE IN THE SCIENTIFIC LIFE AND ANIMATION OF THE TEAM.

Our research focuses on 1.The impact of mechanical stress on endothelial cell homeostasis;

2.New determinants of smooth muscle cell dysfunction, with a particular focus on the PI3K signaling pathway;

3.The preclinical and clinical validation of our findings. These studies aim to identify new cellular targets in order to develop novel therapeutic concepts and strategies for preventing arterial lesions.

MAIN ACTIVITIES

• Carrying out and monitoring in vivo experiments
• Carrying out and monitoring in vitro experiments of endothelial cells cultured under flow
• Analysis and quantification of genes (qRT-PCR)
• Analysis and quantification of proteins (Western Blot, Immunofluorescence)
• Image analysis
• Analysis of transcriptomics data
• Management of murine model breeding
• Ensuring scientific, methodological, and technological monitoring
• Performing graphical presentation of data
• Validating and disseminating results and technological achievements in the form of reports, publications, and oral presentations within the laboratory and at conferences
• Scientifically valorizing results orally and in writing (scientific publications and
• Communications in national and international conferences and symposiums)
• PARTICIPATING IN COLLABORATIVE NETWORK MEETINGS IN ENGLISH (GRAPHICAL PRESENTATION OF DATA AND WRITING MEETING MINUTES) / PARTICIPATING IN COLLABORATIVE NETWORK MEETINGS IN ENGLISH, INCLUDING THE GRAPHICAL PRESENTATION OF DATA AND THE PREPARATION OF MEETING MINUTES

Requirements

• KNOWLEDGE OF ISSUES RELATED TO THE BIOMECHANICAL RESPONSES OF ENDOTHELIAL CELLS IS A PLUS
• MANDATORY CERTIFICATION IN ANIMAL EXPERIMENTATION AND UP-TO-DATE COMPETENCE LOGBOOK

Knowledge

• Good Laboratory Practices
• Knowledge of arterial pathologies and endothelium biology
• Knowledge of hygiene and safety rules
• Knowledge of bioinformatics
• Writing skills (scientific articles) and communication skills (presentations, posters)

Skills

• Using a flow chamber system to model mechanical constraints on cultured endothelial cells
• Performing immunofluorescence/histological techniques on tissue
• Performing gene expression analysis by qRT-PCR and protein analysis by Western-Blot
• Performing image analysis
• Guaranteeing the quality and relevance of analysis tools and results
• Knowing how to plan ...

What we offer

24-MONTH FIXED-TERM CONTRACT / FULL-TIME / 38H30 WEEKLY / REMUNERATION: FROM €2,909.90 GROSS/MONTH, ACCORDING TO THE GRIDS IN FORCE AT INSERM